Pathology Handbook

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CSF for Flow Cytometry

Alternative Names:

Flow Cytometry CSF

Laboratory:

Haematology

Test Code:

Specimen Types:

Cerebrospinal Fluid

Container Types:

RPMI MediaTube 3
RPMI Media Tube 2

Adults Volume or Mass:

2 mL

Minimum/Paediatric Volume or Mass:

2 mL

Collection & Request Instructions:

Collection tubes: collection into specialised media (RPMI) tube 2 or RPMI tube 3

Collection tubes: collection into specialised media (RPMI are available from the pathology laboratory- ring flow lab on ex 63616)

CSF specimen rapidly degrades upon lumbar puncture. Cells combined within a CSF specimen have a short bench life and rapidly die. Immediate addition of RPMI medium and prompt delivery to the Flow Cytometry laboratory (within 2 hours of collection, ideally 30 mins) minimises cell loss.

Transport Instructions between Sites and/or Laboratories:

Room Temperature
If sample cannot be delivered in 30 minutes use ice.

Assay Frequency:

Weekdays