Pathology Handbook

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Mycobacterium tuberculosis PCR

Alternative Names:TBPCR
Laboratory:Microbiology
Test Code:MTBPCR
Specimen Types:Sputum
Bronchoalveolar Lavage
Bronchial Washings
Nasopharyngeal Aspirate
Cerebrospinal Fluid
Container Types:
Plain 70mL pot
CSF Tube 3
Collection & Request Instructions:

Department Head authorisation required

Mycobacterial culture should also be requested

The use of nucleic acid amplification tests (NAAT) for screening specimens from patients with suspected TB should be limited to:

  • Respiratory smear-positive specimens where the result is likely to influence clinical (treatment) and/or public health (isolation, contact investigation) decisions;
  • Respiratory smear-negative specimens from a patient with a high probability of TB, when prompt management and public health decisions are required; and
  • Selected non-respiratory specimens (e.g. meningeal, some tissue biopsies) where a prompt management decision is necessary (recognised that such tests have not been validated or approved).

The use of NAAT is considered inappropriate in the following instances:

  • When a patient is respiratory smear-negative and has a low probability of TB;
  • When a patient is respiratory smear-positive and has a very high probability of TB;
  • Paucibacillary non-respiratory specimens (e.g. pleural fluid, ascitic fluid).
  • Transport Instructions between Sites and/or Laboratories:Room Temperature
    External Laboratory:V.I.D.R.L.
    Additional Notes:

    At least 1 mL of unspun CSF is required for this test.

    Other samples may be tested on request.