Pathology Handbook

PLEASE NOTE: Recent changes have been made to this Test

Recent changes for Factor V Leiden Mutation

Recent changes for Factor V Leiden Mutation
Date	Field	Changed From	Changed To
6th May 2026	Optional Container Type		EDTA 3 mL
5th May 2026	Frequency	Monthly	Weekly
5th May 2026	Alternative names	FVLM, FV Leiden, Leiden Mutation, Activated Protein C Resistance Mutation Testing	FVLM, FV Leiden, Leiden Mutation, Activated Protein C Resistance Mutation Testing, F5L
	Volume (Adults)		2.7 mL
	Minimum/Paediatric Volume		1.0 mL
	Preferred Container Type	EDTA 10 mL	Sodium Citrate

Factor V Leiden Mutation

Alternative Names:

FVLM
FV Leiden
Leiden Mutation
Activated Protein C Resistance Mutation Testing
F5L

Laboratory:

Human Molecular

Test Code:

FVLM

Specimen Types:

Blood

Container Types:

Sodium Citrate
EDTA 3 mL

Adults Volume or Mass:

2.7 mL

Minimum/Paediatric Volume or Mass:

1 mL

Collection & Request Instructions:

For identification of carriers of inherited venous thrombophilia. General population screening and routine screening before prescribing oral contraceptives or hormone replacement therapy are not recommended. test should only be requested by a practioner skilled in genetic counselling of thrombophilia. The significance of a polymorphism in an affected individual is uncertain. Test is Factor -V-Leiden G1691A or R506Q.

PCR assay for detection of Factor V nucleotide substitution causing Activated Protein C Resistance.

Transport Instructions between Sites and/or Laboratories:

Room Temperature

Assay Frequency:

Weekly

External Laboratory:

Melbourne Health Shared Pathology Service

Additional Notes:

Check to see if Factor 5 level or Factor 5 leiden - often confused and ordered incorrectly

Pathology Handbook

PLEASE NOTE: Recent changes have been made to this Test

Optional Container Type

Frequency

Alternative names

Volume (Adults)

Minimum/Paediatric Volume

Preferred Container Type

Factor V Leiden Mutation