Pathology Handbook

Recent changes for Paroxysmal Nocturnal Hemoglobinuria Phenotype
Date	Field	Changed From	Changed To
29th June 2026	Request Group	PNH	PNHFLOW
29th June 2026	Alternative names	PNH	PNH, CD55 & CD59, PNH by flow, FLAER for PNH
	Minimum/Paediatric Volume		1 mL
	Preferred Container Type	EDTA 3 mL	Lithium Heparin (without Gel)
	Optional Container Type	Lithium Heparin (without Gel)
	Collection & Request Instructions	Sample should be taken before transfusion. Specimens must be kept at room temperature and must be less than 24hours old. Specimens should be sent to the Haematology Laboratory on Mondays to Thursdays between 0900-1730 hrs. Outside these hours liase with the Haemtology Registrar on ext 63075 before collecting.	Sample should be taken before transfusion. Specimens must be kept at room temperature and must be less than 48 hours old. Specimens should be collected to arrive at the Clayton Immunology department on Monday to Friday between 0900-1730 hrs or Saturday 0900-1300 If sample will arrive outside these hours liase with the laboratory on 03 9594 3587 before collecting.
	Frequency	Weekly	Daily

Alternative Names:

PNH
CD55 & CD59
PNH by flow
FLAER for PNH

Laboratory:

Haematology

Test Code:

PNHFLOW

Specimen Types:

Blood

Container Types:

Lithium Heparin (without Gel)

Adults Volume or Mass:

3 mL

Minimum/Paediatric Volume or Mass:

1 mL

Collection & Request Instructions:

Sample should be taken before transfusion.

Specimens must be kept at room temperature and must be less than 48 hours old.

Specimens should be collected to arrive at the Clayton Immunology department on Monday to Friday between 0900-1730 hrs or Saturday 0900-1300

If sample will arrive outside these hours liase with the laboratory on 03 9594 3587 before collecting.

Transport Instructions between Sites and/or Laboratories:

Room Temperature

Assay Frequency:

Daily

External Laboratory:

Monash Pathology

Additional Notes:

Sample should be taken before transfusion.

Specimens must be kept at room temperature and must be less than 48 hours old.

Specimens should be collected to arrive at the Clayton Immunology department on Monday to Friday between 0900-1730 hrs or Saturday 0900-1300

If sample will arrive outside these hours liase with the laboratory on 03 9594 3587 before collecting.