Collection tubes: collection into specialised media (RPMI) tube 2 or RPMI tube 3
Collection tubes: collection into specialised media (RPMI are available from the pathology laboratory- ring flow lab on ex 63616)
CSF specimen rapidly degrades upon lumbar puncture. Cells combined within a CSF specimen have a short bench life and rapidly die. Immediate addition of RPMI medium and prompt delivery to the Flow Cytometry laboratory (within 2 hours of collection, ideally 30 mins) minimises cell loss.