Pathology Handbook

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Total Haemolytic Complement

Alternative Names:

Haemolytic Complement; Total
CH100
CH50
classical pathway
AH50
Alternate pathway
Mannose pathway

Laboratory:

Haematology

Test Code:

THC

Specimen Types:

Serum

Container Types:

Plain tube - serum (no Gel)

Adults Volume or Mass:

3 mL

Collection & Request Instructions:

Collect at ambient temperature

Samples should be processed as “urgent” as Functional complement tests have precise collection requirements to ensure accurate and reproducible results.Plasma, heat inactivated serum or sera with pronounced hemolysis, icterus, lipemia or microbial growth are not suitable for testing and will not be processed

Collect 3 ml of whole blood into plain gel-free (neutral) serum tube- time critical - take to lab for processing of sample.

Transport Instructions between Sites and/or Laboratories:

On Ice

Transport Instructions between Sites and/or Laboratories:

Dry ice

Transport the 3 aliquots to RCH on dry ice.

Do not allow samples to thaw before or during transport.

Assay Frequency:

Fortnightly

External Laboratory:

Women's and Children's Pathology Services, RCH

Additional Notes:

Very time dependent collection and processing required- 3 ml of whole blood into plain gel-free (neutral) serum tube required.

Take immediately to pathology and label as urgent