Pathology Handbook

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PLEASE NOTE: Recent changes have been made to this Test

Recent changes for Alzheimer

Recent changes for Alzheimer
Date	Field	Changed From	Changed To
19th February 2024	Collection & Request Instructions	Must be approved by a Chemical Pathologist. Lumber puncture. Prefererably before Noon. Collect 1-2 mL CSF in standard collection tubes for glucose and protein and cell counts. Perform testing at The Alfred Collect 2.5 mL of clear, non-haemolysed CSF into a blue capped PP tube. If reddish, collect aditional CSF into a new tube. Send tube(s) to NDDL DO NOT FREEZE CSF https://pathology.alfred.org.au/handbook/downloads/clinical-biochemistry/FRM-AD_CJD-002%20V1%20220720.pdf Please complete NDDL referral form: https://pathology.alfred.org.au/handbook/downloads/clinical-biochemistry/FRM-AD_CJD-001%20V1%20220720.pdf	Must be approved by a Chemical Pathologist. Lumber puncture. Prefererably before Noon. Collect 1-2 mL CSF in standard collection tubes for glucose and protein and cell counts. Perform testing at The Alfred Collect 2.5 mL of clear, non-haemolysed CSF into a blue capped PP tube. If reddish, collect aditional CSF into a new tube. Send tube(s) to NDDL DO NOT FREEZE CSF The NDDL referral form must be completed prior to referral.
28th November 2023	Frequency	Fortnightly	Weekly

Date

Field

Changed From

Changed To

19th February 2024

Collection & Request Instructions

Must be approved by a Chemical Pathologist.

Lumber puncture. Prefererably before Noon.

Collect 1-2 mL CSF in standard collection tubes for glucose and protein and cell counts. Perform testing at The Alfred

Collect 2.5 mL of clear, non-haemolysed CSF into a blue capped PP tube. If reddish, collect aditional CSF into a new tube. Send tube(s) to NDDL

DO NOT FREEZE CSF

https://pathology.alfred.org.au/handbook/downloads/clinical-biochemistry/FRM-AD_CJD-002%20V1%20220720.pdf

Please complete NDDL referral form:

https://pathology.alfred.org.au/handbook/downloads/clinical-biochemistry/FRM-AD_CJD-001%20V1%20220720.pdf

Must be approved by a Chemical Pathologist.

Lumber puncture. Prefererably before Noon.

Collect 1-2 mL CSF in standard collection tubes for glucose and protein and cell counts. Perform testing at The Alfred

Collect 2.5 mL of clear, non-haemolysed CSF into a blue capped PP tube. If reddish, collect aditional CSF into a new tube. Send tube(s) to NDDL

DO NOT FREEZE CSF

The NDDL referral form must be completed prior to referral.

28th November 2023

Frequency

Fortnightly

Weekly

Alzheimer

Alternative Names:

Abeta
Tau

Laboratory:

Clinical Biochemistry

Test Code:

ALZHEIM

Specimen Types:

Cerebrospinal Fluid

Container Types:

CSF Tube (blue capped PP)

Adults Volume or Mass:

5 mL

Minimum/Paediatric Volume or Mass:

5 mL

Collection & Request Instructions:

Must be approved by a Chemical Pathologist.

Lumber puncture. Prefererably before Noon.

Collect 1-2 mL CSF in standard collection tubes for glucose and protein and cell counts. Perform testing at The Alfred

Collect 2.5 mL of clear, non-haemolysed CSF into a blue capped PP tube. If reddish, collect aditional CSF into a new tube. Send tube(s) to NDDL

DO NOT FREEZE CSF

The NDDL referral form must be completed prior to referral.

Transport Instructions between Sites and/or Laboratories:

RoomTemp within Alfred/Esky from External sites

Assay Frequency:

Weekly

External Laboratory:

National Dementia Diagnostics Laboratory

Additional Notes:

Reference Intervals

CSF marker	Normal Reference (ng/L)
Abeta1-42	> 1030
Phospho-tau (P-tau)181	≤ 27
Total-tau (T-tau)	≤ 300
P-tau/Abeta1-42	≤ 0.023
T-tau/Abeta1-42	≤ 0.28

The CSF biomarkers Abeta1-42 and P-tau levels and the P-tau/Abeta1-42 ratio offer approximately 85-95% predictive value for
supporting underlying Alzheimer's disease neuropathologic changes based on Abeta amyloid-PET imaging (1).

The normal reference ranges are based on strictly defined pre-analytical handling parameters for CSF specimens. Failure to adhere to
the collection instructions may result in falsely low Abeta1-42 concentrations, affecting the interpretation (2).
*Note: As of 28/02/2022, the Roche Elecsys Immunoassay Generation II for CSF is used for testing.

The phone number to discuss this report is 03 9035 7243 (NDDL)

1. Hansson et al.,Alzheimer's & Dementia 2018; 14: 1470; Schindler et al. Alzheimer’s & Dementia 14 (2018) 1460.
2. https://pim-eservices.roche.com/eLD/api/downloads/7aa19107-2bbd-eb11-0391-005056a772fd?countryIsoCode=au