Pathology Handbook

PLEASE NOTE: Recent changes have been made to this Test

Recent changes for Alzheimer's Disease Screen

Recent changes for Alzheimer's Disease Screen
Date	Field	Changed From	Changed To
6th May 2022	Collection & Request Instructions	Must be approved by a Chemical Pathologist. Lumber puncture. Prefererably before Noon. Collect 1-2 mL CSF in standard collection tubes for glucose and protein and cell counts. Perform testing at The Alfred Collect 2.5 mL of clear, non-haemolysed CSF into a blue capped PP tube. If reddish, collect aditional CSF into a new tube. Send tube(s) to NDDL https://pathology.alfred.org.au/handbook/downloads/clinical-biochemistry/FRM-NDD-002%20NDDL%20CSF%20protocol%20GII%20V11%20220228.pdf	Must be approved by a Chemical Pathologist. Lumber puncture. Prefererably before Noon. Collect 1-2 mL CSF in standard collection tubes for glucose and protein and cell counts. Perform testing at The Alfred Collect 2.5 mL of clear, non-haemolysed CSF into a blue capped PP tube. If reddish, collect aditional CSF into a new tube. Send tube(s) to NDDL https://pathology.alfred.org.au/handbook/downloads/clinical-biochemistry/FRM-NDD-002%20NDDL%20CSF%20protocol%20GII%20V11%20220228.pdf DO NOT FREEZE CSF Please complete NDDL referral form: https://pathology.alfred.org.au/handbook/downloads/clinical-biochemistry/FRM-NDD-001%20AD%20CSF%20Assays%20Specimen%20Data%20sheet%20v16%20220308.pdf
6th May 2022	Collection & Request Instructions	Lumber puncture. Prefererably before Noon. Collect 1-2 mL CSF in standard collection tubes for glucose and protein and cell counts. Perform testing at The Alfred Collect 2.5 mL of clear, non-haemolysed CSF into a blue capped PP tube. If reddish, collect aditional CSF into a new tube. Send tube(s) to NDDL https://pathology.alfred.org.au/handbook/downloads/clinical-biochemistry/FRM-NDD-002%20NDDL%20CSF%20protocol%20GII%20V11%20220228.pdf	Must be approved by a Chemical Pathologist. Lumber puncture. Prefererably before Noon. Collect 1-2 mL CSF in standard collection tubes for glucose and protein and cell counts. Perform testing at The Alfred Collect 2.5 mL of clear, non-haemolysed CSF into a blue capped PP tube. If reddish, collect aditional CSF into a new tube. Send tube(s) to NDDL https://pathology.alfred.org.au/handbook/downloads/clinical-biochemistry/FRM-NDD-002%20NDDL%20CSF%20protocol%20GII%20V11%20220228.pdf
6th May 2022	Assay name	Alzheimer	Alzheimer's Disease Screen
	Collection & Request Instructions	Lumber puncture. Prefererably before Noon. Collect 1-2 mL CSF in standard collection tubes for glucose and protein and cell counts. Perform testing at The Alfred Collect 2.5 mL of clear, non-haemolysed CSF into a blue capped PP tube. If reddish, collect aditional CSF into a new tube. Send tube(s) to NDDL	Lumber puncture. Prefererably before Noon. Collect 1-2 mL CSF in standard collection tubes for glucose and protein and cell counts. Perform testing at The Alfred Collect 2.5 mL of clear, non-haemolysed CSF into a blue capped PP tube. If reddish, collect aditional CSF into a new tube. Send tube(s) to NDDL https://pathology.alfred.org.au/handbook/downloads/clinical-biochemistry/FRM-NDD-002%20NDDL%20CSF%20protocol%20GII%20V11%20220228.pdf
6th May 2022	Assay name	Alzheimer's Disease Screen	Alzheimer
6th May 2022	Assay name	Alzheimer	Alzheimer's Disease Screen
6th May 2022	Assay name	Alzheimer's Disease Screen	Alzheimer
	Preferred Container Type	CSF Tube 1	CSF Tube (blue capped PP)
4th May 2022	Assay name	Alzheimer	Alzheimer's Disease Screen

Alzheimer's Disease Screen

Alternative Names:

Abeta
Tau

Laboratory:

Clinical Biochemistry

Test Code:

ALZHEIM

Specimen Types:

Cerebrospinal Fluid

Container Types:

CSF Tube (blue capped PP)

Adults Volume or Mass:

5 mL

Minimum/Paediatric Volume or Mass:

5 mL

Collection & Request Instructions:

Must be approved by a Chemical Pathologist.

Lumber puncture. Prefererably before Noon.

Collect 1-2 mL CSF in standard collection tubes for glucose and protein and cell counts. Perform testing at The Alfred

Collect 2.5 mL of clear, non-haemolysed CSF into a blue capped PP tube. If reddish, collect aditional CSF into a new tube. Send tube(s) to NDDL

https://pathology.alfred.org.au/handbook/downloads/clinical-biochemistry/FRM-NDD-002%20NDDL%20CSF%20protocol%20GII%20V11%20220228.pdf

DO NOT FREEZE CSF

Please complete NDDL referral form:

https://pathology.alfred.org.au/handbook/downloads/clinical-biochemistry/FRM-NDD-001%20AD%20CSF%20Assays%20Specimen%20Data%20sheet%20v16%20220308.pdf

Transport Instructions between Sites and/or Laboratories:

RoomTemp within Alfred/Esky from External sites

Assay Frequency:

Fortnightly

External Laboratory:

National Dementia Diagnostics Laboratory

Additional Notes:

Reference Intervals

CSF marker	Normal Reference (ng/L)
Abeta1-42	> 1030
Phospho-tau (P-tau)181	≤ 27
Total-tau (T-tau)	≤ 300
P-tau/Abeta1-42	≤ 0.023
T-tau/Abeta1-42	≤ 0.28

The CSF biomarkers Abeta1-42 and P-tau levels and the P-tau/Abeta1-42 ratio offer approximately 85-95% predictive value for
supporting underlying Alzheimer's disease neuropathologic changes based on Abeta amyloid-PET imaging (1).

The normal reference ranges are based on strictly defined pre-analytical handling parameters for CSF specimens. Failure to adhere to
the collection instructions may result in falsely low Abeta1-42 concentrations, affecting the interpretation (2).
*Note: As of 28/02/2022, the Roche Elecsys Immunoassay Generation II for CSF is used for testing.

The phone number to discuss this report is 03 9035 7243 (NDDL)

1. Hansson et al.,Alzheimer's & Dementia 2018; 14: 1470; Schindler et al. Alzheimer’s & Dementia 14 (2018) 1460.
2. https://pim-eservices.roche.com/eLD/api/downloads/7aa19107-2bbd-eb11-0391-005056a772fd?countryIsoCode=au

Pathology Handbook

PLEASE NOTE: Recent changes have been made to this Test

Collection & Request Instructions

Collection & Request Instructions

Assay name

Collection & Request Instructions

Assay name

Assay name

Assay name

Preferred Container Type

Assay name

Alzheimer's Disease Screen