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PLEASE NOTE: Recent changes have been made to this Test


 Recent changes for Alzheimer's Disease Screen

Recent changes for Alzheimer's Disease Screen
DateFieldChanged FromChanged To
6th May 2022
Collection & Request Instructions

Must be approved by a Chemical Pathologist.

Lumber puncture.  Prefererably before Noon.

Collect 1-2 mL CSF in standard collection tubes for glucose and protein and cell counts.  Perform testing at The Alfred

Collect 2.5 mL of clear, non-haemolysed CSF into a blue capped PP tube.  If reddish, collect aditional CSF into a new tube.  Send tube(s) to NDDL

https://pathology.alfred.org.au/handbook/downloads/clinical-biochemistry/FRM-NDD-002%20NDDL%20CSF%20protocol%20GII%20V11%20220228.pdf

Must be approved by a Chemical Pathologist.

Lumber puncture.  Prefererably before Noon.

Collect 1-2 mL CSF in standard collection tubes for glucose and protein and cell counts.  Perform testing at The Alfred

Collect 2.5 mL of clear, non-haemolysed CSF into a blue capped PP tube.  If reddish, collect aditional CSF into a new tube.  Send tube(s) to NDDL

https://pathology.alfred.org.au/handbook/downloads/clinical-biochemistry/FRM-NDD-002%20NDDL%20CSF%20protocol%20GII%20V11%20220228.pdf

DO NOT FREEZE CSF

Please complete NDDL referral form:

https://pathology.alfred.org.au/handbook/downloads/clinical-biochemistry/FRM-NDD-001%20AD%20CSF%20Assays%20Specimen%20Data%20sheet%20v16%20220308.pdf

6th May 2022
Collection & Request Instructions

Lumber puncture.  Prefererably before Noon.

Collect 1-2 mL CSF in standard collection tubes for glucose and protein and cell counts.  Perform testing at The Alfred

Collect 2.5 mL of clear, non-haemolysed CSF into a blue capped PP tube.  If reddish, collect aditional CSF into a new tube.  Send tube(s) to NDDL

https://pathology.alfred.org.au/handbook/downloads/clinical-biochemistry/FRM-NDD-002%20NDDL%20CSF%20protocol%20GII%20V11%20220228.pdf

Must be approved by a Chemical Pathologist.

Lumber puncture.  Prefererably before Noon.

Collect 1-2 mL CSF in standard collection tubes for glucose and protein and cell counts.  Perform testing at The Alfred

Collect 2.5 mL of clear, non-haemolysed CSF into a blue capped PP tube.  If reddish, collect aditional CSF into a new tube.  Send tube(s) to NDDL

https://pathology.alfred.org.au/handbook/downloads/clinical-biochemistry/FRM-NDD-002%20NDDL%20CSF%20protocol%20GII%20V11%20220228.pdf

6th May 2022
Assay name

Alzheimer

Alzheimer's Disease Screen

Collection & Request Instructions

Lumber puncture.  Prefererably before Noon.

Collect 1-2 mL CSF in standard collection tubes for glucose and protein and cell counts.  Perform testing at The Alfred

Collect 2.5 mL of clear, non-haemolysed CSF into a blue capped PP tube.  If reddish, collect aditional CSF into a new tube.  Send tube(s) to NDDL

Lumber puncture.  Prefererably before Noon.

Collect 1-2 mL CSF in standard collection tubes for glucose and protein and cell counts.  Perform testing at The Alfred

Collect 2.5 mL of clear, non-haemolysed CSF into a blue capped PP tube.  If reddish, collect aditional CSF into a new tube.  Send tube(s) to NDDL

https://pathology.alfred.org.au/handbook/downloads/clinical-biochemistry/FRM-NDD-002%20NDDL%20CSF%20protocol%20GII%20V11%20220228.pdf

6th May 2022
Assay name

Alzheimer's Disease Screen

Alzheimer

6th May 2022
Assay name

Alzheimer

Alzheimer's Disease Screen

6th May 2022
Assay name

Alzheimer's Disease Screen

Alzheimer

Preferred Container Type

CSF Tube 1

CSF Tube (blue capped PP)

4th May 2022
Assay name

Alzheimer

Alzheimer's Disease Screen

Alzheimer's Disease Screen

Alternative Names:Abeta
Tau
Laboratory:Clinical Biochemistry
Test Code:ALZHEIM
Specimen Types:Cerebrospinal Fluid
Container Types:
CSF Tube (blue capped PP)
Adults Volume or Mass:5 mL
Minimum/Paediatric Volume or Mass:5 mL
Collection & Request Instructions:

Must be approved by a Chemical Pathologist.

Lumber puncture.  Prefererably before Noon.

Collect 1-2 mL CSF in standard collection tubes for glucose and protein and cell counts.  Perform testing at The Alfred

Collect 2.5 mL of clear, non-haemolysed CSF into a blue capped PP tube.  If reddish, collect aditional CSF into a new tube.  Send tube(s) to NDDL

https://pathology.alfred.org.au/handbook/downloads/clinical-biochemistry/FRM-NDD-002%20NDDL%20CSF%20protocol%20GII%20V11%20220228.pdf

DO NOT FREEZE CSF

Please complete NDDL referral form:

https://pathology.alfred.org.au/handbook/downloads/clinical-biochemistry/FRM-NDD-001%20AD%20CSF%20Assays%20Specimen%20Data%20sheet%20v16%20220308.pdf

Transport Instructions between Sites and/or Laboratories:RoomTemp within Alfred/Esky from External sites
Assay Frequency:Fortnightly
External Laboratory:National Dementia Diagnostics Laboratory
Additional Notes:

Reference Intervals

  CSF marker

  Normal Reference (ng/L)

  Abeta1-42

  > 1030

  Phospho-tau (P-tau)181

  ≤ 27

  Total-tau (T-tau)

  ≤ 300

  P-tau/Abeta1-42

  ≤ 0.023

  T-tau/Abeta1-42

  ≤ 0.28


The CSF biomarkers Abeta1-42 and P-tau levels and the P-tau/Abeta1-42 ratio offer approximately 85-95% predictive value for
supporting underlying Alzheimer's disease neuropathologic changes based on Abeta amyloid-PET imaging (1).

The normal reference ranges are based on strictly defined pre-analytical handling parameters for CSF specimens. Failure to adhere to
the collection instructions may result in falsely low Abeta1-42 concentrations, affecting the interpretation (2).
*Note: As of 28/02/2022, the Roche Elecsys Immunoassay Generation II for CSF is used for testing.

The phone number to discuss this report is 03 9035 7243 (NDDL)

 

1. Hansson et al.,Alzheimer's & Dementia 2018; 14: 1470; Schindler et al. Alzheimer’s & Dementia 14 (2018) 1460.
2. https://pim-eservices.roche.com/eLD/api/downloads/7aa19107-2bbd-eb11-0391-005056a772fd?countryIsoCode=au