Pathology Handbook

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Anatomical Pathology Shared Specimen Procedures

Alternative Names:Shared Specimens (Micro & Anat Path)
Laboratory:Anatomical Pathology
Test Code:Shared
Registration Instructions:
NORMAL HOURS
SPECIMEN
HANDLING
ANATOMICAL PATHOLOGY SPECIMENS
Specimens marked “Urgent”
Deliver to AP Urgently
Fresh tissue marked for Frozen section
Deliver to AP Urgently
Specimen for Immunofluorescence
Deliver to AP Urgently
CSF for Cytology
Deliver to AP Urgently (may be a shared sample )
Other Fresh tissue (i.e. not in formalin)
Deliver to AP as a Priority
Other Cytology specimens (fluids, sputum etc)
For delivery by Internal Courier
Blood specimens (Autoantibodies)
For delivery by Internal Courier
Formalin fixed & All other specimens
For delivery by Internal Courier
MICROBIOLOGY SPECIMENS
Specimens to be shared
Deliver to micro Urgently
Urgent specimens
Deliver to micro Urgently
All other specimens
For delivery by CSP & micro staff
 
AFTER HOURS
SPECIMEN
HANDLING
ANATOMICAL PATHOLOGY SPECIMENS
Sample marked Urgent
CSP to Contact On-Call Anatomical Pathologist
Fresh tissue marked for Frozen section
CSP to Contact On-Call Anatomical Pathologist
Fridge 2-8° C Anat Path box as interim
Specimen for Immunofluorescence
Fridge 2-8° C Anat Path box
CSF for Cytology (if Microbiology staffed)
Deliver to micro urgently. Micro will cytospin
CSF for cytology (if microbiology closed)
If shared specimen: Micro Room Temp. racks
If Cyto only: Fridge 2-8° C Anat Path box
Other Fresh tissue (i.e. not in formalin)
Fridge 2-8° C Anat Path box
Other Cytology specimens (fluids, sputum etc)
Fridge 2-8° C Anat Path box
Blood specimens (AP Autoantibodies))
Centrifuge and Fridge 2-8° C
Anat Path box
Formalin fixed
Room temp- Anat Path box
If in doubt about the urgency or handling of a specimen
Contact On-Call Anatomical Pathologist
(Avoid calls between 10pm and 7am)
Fridge 2-8° C Anat Path box as interim
MICROBIOLOGY SPECIMENS
Blood Cultures/Paediatric Cultures
Incubator 
 
 
Blood Pack Cells
Micro Room Temp. racks
HIV Viral Load/ HIVVGM
Micro Room Temp. racks
Quanitferon
Micro Room Temp. racks
Spinal Fluid (CSF)
Micro Room Temp. racks
Swabs
Micro Room Temp. racks
AFB Blood cultures
Micro Room Temp. racks
 
 
Catheter Tips
Fridge 2-8° C
CMV Viral Load Blood
Fridge 2-8° C
EBVLOAD
Fridge 2-8° C
Faeces
Fridge 2-8° C
Fluids/Aspirates
Fridge 2-8° C
Gono PCR swabs
Fridge 2-8° C
Serology
Fridge 2-8° C
Sputum / BAL
Fridge 2-8° C
Urine
Fridge 2-8° C
VTM’s
Fridge 2-8° C
Fresh tissue
Fridge 2-8° C
 
Specimen Types:See Under Collection Instructions
Container Types:
See Detailed Information
Processing Instructions:
NORMAL HOURS
Anatomical Pathology Specimens (Tissue, Fluids & Bloods)
Non-Shared Specimen:
i.e. No Microbiology or Immunology requests on the same specimen
Formalin fixed tissue specimens, fresh tissue specimens and cytology specimens: Write up on AP log sheet and deliver to Anatomical Pathology by Internal Courier.
Blood tubes: Blood specimens for autoantibody serology performed in Anatomical Pathology (ANA, ANCA, Adrenal, GPC, Heart, LKM, Mitochondrial, Neuronal, skeletal muscle, striated and smooth muscle) are daybooked and centrifuged before being written up on AP log sheet and delivered by Internal Courier.
Urgent Processing: Any Specimen marked “Urgent” or “Frozen Section” or “Immunofluorescence” and CSF specimens for cytology. These should be delivered to Anatomical Pathology immediately. Call the histology laboratory (extn 62476) to collect if necessary.
 
Shared Specimen with Microbiology: 
i.e. Microbiology request on the same specimen
Cytology Fluids: Deliver whole specimen to Microbiology first.
Tests for micro are daybooked, as well as ‘CYTO’ for specimens unable to be split. Specimen and request are labelled with a “specimen to be shared” sticker. AP receives a copy of the request marked as “Slip Only” (delivered by Internal Courier). It is documented on the AP log sheet.
Fresh Tissue: Deliver whole specimen to Anatomical Pathology first.
Specimen and request are labelled with a “specimen to be shared” sticker. A Pathologist will assess and divide the specimen to provide a sample for Microbiology.The sample for Microbiology will be returned by Anatomical Pathology to CSP for daybooking.
Shared Specimen with Immunology
i.e. Immunology (eg Flowcytometry or AA’s) request on the same specimen
Fresh tissue: Deliver whole specimen to Anatomical Pathology first.
A Pathologist will divide the specimen to provide a sample for Immunology.The sample for Immunology will be returned by Anatomical Pathology to CSP for daybooking.
Blood tubes: Some autoantibody serology is performed in Anatomical Pathology (ANA, ANCA, Adrenal, GPC, Heart, LKM, Mitochondrial, Neuronal, skeletal muscle, striated and smooth muscle). Blood specimens are daybooked and centrifuged before being written up on AP log sheet and delivered by Internal Courier.
Microbiology Specimens
Refer to table.
AFTER HOURS
Anatomical Pathology Specimens
If any specimen received after hours is marked “Urgent” or “Frozen Section” please contact the On-Call Anatomical Pathologist through Switchboard. This should happen rarely; urgent specimens are generally discussed with the Pathologist beforehand (if possible avoid calls between 10 pm and 7 am).
Formalin fixed tissue: After hours store all fixed specimens at room temperature (NB: Do not place formalin specimens in the fridge).
Fresh tissue:  After hours contact the on-call Pathologist or Scientist (NB avoid calls between 10pm to 7am if possible). Store sample in the refrigerator.
Cytology specimens: After hours store in the refrigerator. If a CSF see below;
CSF Specimen:           
Outside Cytology Hours but within Microbiology Hours daybook as CYTO and pass to Microbiology who will prepare the Cytospin smears for Cytology
(NB. Between 5pm and 5:30pm please contact Cytology on Extn 63088 ASAP to advise them a CSF has been received). If notified Cytology will collect and process.
Outside Microbiology Hours daybook as CYTO and store in the refrigerator in the AP box.
 
Shared Specimen with Microbiology:
 i.e. Microbiology request(s) on the same specimen.
Fresh tissue: Treat the same as AP shared specimens in normal hours and store in the refrigerator in the AP box.
CSF Specimen: Store at room temperature in the Micro rack. Tests for micro are daybooked, as well as ‘CYTO’ for specimens unable to be split. Specimen and request are labelled with a “specimen to be shared” sticker. Store CSF specimen at room temperature in the Micro rack. AP receives a copy of the request marked “Slip only” (delivered by Internal Courier). It is documented on the AP log sheet.
 
 Shared Specimen with Immunology:
 i.e. Immunology request(s) on the same specimen (eg request says Flowcytometry, T&B cells, phenotyping, lymphoma) or if blood tubes for autoantibody testing.
Fresh tissue: If specimen arrives after hours contact the On-Call Pathologist (NB avoid calls between 10pm to 7am if possible). Store in refrigerator in the interim.
Blood tube:  Daybook, centrifuge and store in refrigerator.
Transport Instructions between Sites and/or Laboratories:See Detailed Information