PCR can be used to detect fungal DNA in a variety of clinical specimens including tissue, CSF, BAL and vitreous fluid. ICPMR does not perform Panfungal PCR on sputum and blood samples.
PCR can be used to identify fungi from pure culture. It is particularly helpful to identify risk group 3 pathogens, slow growing or poorly sporulating moulds.
Sample requirements:
Fresh tissue or 10x10um paraffin embedded tissue sections in a sterile container, 0.5 mL CSF, 1 mL BAL, 0.2 mL vitreous fluid.
Culture: pure fungal culture |