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Fungal PCR

Alternative Names:Pan Fungal PCR
Laboratory:Microbiology
Test Code:PANFUNGPCR
Specimen Types:Tissue
Bronchoalveolar Lavage
Bronchial Washings
Cerebrospinal Fluid
Container Types:
Plain 70mL pot
CSF Tube 3
Adults Volume or Mass:1 mL
Collection & Request Instructions:

Department head authorisation required. (ID consult team exempt).

Processing Instructions:

PCR can be used to detect fungal DNA in a variety of clinical specimens including tissue, CSF, BAL and vitreous fluid. ICPMR does not perform Panfungal PCR on sputum and blood samples.

PCR can be used to identify fungi from pure culture. It is particularly helpful to identify risk group 3 pathogens, slow growing or poorly sporulating moulds.

Sample requirements:

Fresh tissue or 10x10um paraffin embedded tissue sections in a sterile container, 0.5 mL CSF, 1 mL BAL, 0.2 mL vitreous fluid.

Culture: pure fungal culture

Transport Instructions between Sites and/or Laboratories:Room Temperature

Check Fungal culture result PRIOR to sending sample.

DO NOT send if culture positive for filamentous fungi or yeast.

Assay Frequency:Fortnightly
External Laboratory:NSW Health Pathology, Westmead (ICPMR)
External Transport Instructions:Send at ambient temperature