Pathology Handbook

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Creutzfeldt-Jacob Disease Protein

Alternative Names:

CJD Protein
Protein 14-3-3

Laboratory:

Microbiology

Test Code:

CJD

Specimen Types:

Cerebrospinal Fluid

Container Types:

CSF Tube 3

Adults Volume or Mass:

1 mL

Collection & Request Instructions:

Department Head authorisation required (except Neurology patients).

Processing Instructions:

Do Not Centrifuge

CSF must be clear and colourless, not spun

14-3-3 ELISA: Red cell count: <2500 x 10⁶/L and non-haemolysed.

RT-QulC: Red cell count <1250 x 10⁶/L; White cell count <10 x 10⁶/L

Cell count must be performed on the tube to be sent (or a higher numbered tube).

CSF should be transferred (unspun) into a labelled red screw top container using a sterile pipette as soon as possible after collection. Parafilm the lid. Initial label to indicate patient details have been checked.

Place red screw top container in a 50mL screw top centrifuge tube and parafilm the lid

Freeze specimen at -80'C and store frozen. Record tube used and the date and time of freezing on the NDDL request form accompanying the specimen.

Samples can be stored at 4^oC for up to 4 days.

Transport Instructions between Sites and/or Laboratories:

On Ice

Assay Frequency:

Weekly

External Laboratory:

CJD Registry

External Transport Instructions:

Send Frozen on Dry Ice

Transport in a biohazard bag and then place in a second biohazard bag.

Transport on dry ice, refer to CD_MI_0369_Serology_Sendaway_Procedure.doc for instructions.

Fill out 14-3-3 specimen data form and include biochem results and cell count.

Notify CJD Registry on (03) 8344 1949 before sending.

Specimen Data Sheet and general instructions can be found here

https://florey.edu.au/wp-content/uploads/2024/05/NDDL-Specimen-Data-sheet-May-2024.pdf