Pathology Handbook

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Interferon Gamma

Alternative Names:QuantiFERON Monitor
Laboratory:Microbiology
Test Code:QUANTMON
Specimen Types:Plasma
Container Types:
See Under Collection Instructions
Collection & Request Instructions:

This test is only available as part of a clinical trial.

A white-capped QuantiFERON tube is required.  These are supplied directly to the Trial Co-ordinator.

The blood collection tubes supplied are for use only with the QuantiFERON®-Monitor system (QFT®). The following instructions relate solely to the use of QFT® blood collection tubes.

Gently invert the tubes after collection to ensure the heparin in the tube is dissolved. The tubes must be transferred to a 37oC incubator as soon as possible and within 16 hours of collection.

 Note: Samples must reach the laboratory by 1600 Monday to Friday for processing.

DO NOT SEND on weekends or public holidays.

The following procedures should be followed for optimal results:

 

Blood Collection:

For each subject collect 1mL of blood by venipuncture directly into the QFT® blood collection tube.

As 1mL tubes draw blood relatively slowly, keep the tube on the needle for 2-3 seconds once the tube appears to have completed filling, to ensure that the correct volume is drawn.

The black mark on the side of the tubes indicates the 1mL fill volume. QFT® blood collection tubes have been validated for volumes ranging from 0.8 to 1.2 mL. If the level of blood in any tube is not close to the indicator line, it is recommended to obtain another blood sample.

If a “butterfly needle” is used, prime tubing with a “purge” tube before filling the QFT® tubes.

Mix to ensure that the entire inner surface of the tube has been coated with the blood.

Thorough mixing is required to ensure complete integration of the tube’s contents into the blood.

 

Label tubes appropriately.

Transport Instructions between Sites and/or Laboratories:Room Temperature
Assay Frequency:Fortnightly